RNA Mass Moles Converter

Convert between mass and moles for ssRNA and dsRNA sequences

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Set up your conversion parameters for RNA
RNA Strand Type

Applied to each strand; 5′ triphosphate is common for in-vitro transcripts.

Maximum 5,000,000 bases.

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Calculation Formulas & Scientific Background
Mathematical formulas and molecular biology principles used for mass to moles conversion for RNA

Mass to Moles Conversion Formulas

1. Calculate Moles of RNA

moles of RNA [mol] = RNA mass [g] / RNA molecular weight [g/mol]

This equation converts the physical mass of RNA to the number of moles using the molecular weight.

2. Calculate Moles of RNA Ends

moles of ssRNA ends [mol] = moles of ssRNA [mol] × 1

Each single-stranded RNA molecule has one 3' and one 5' end counted as one end unit for ligation calculations.

3. Calculate RNA Copy Number

RNA copy number [molecules] = moles of RNA [mol] × 6.022e23 [molecules/mol]

Uses Avogadro's number to convert moles to actual number of RNA molecules.

4. Calculate RNA Molecular Weight

MW = strand residue sum − 61.96 + selected 5′ adjustment

When a sequence is provided, A/U and C/G complements are calculated explicitly. Length-only estimates use an average RNA residue weight of approximately 321.44 g/mol per nucleotide.